|
KMID : 0665220190320010033
|
|
Korean Journal of Food and Nutrition
2019 Volume.32 No. 1 p.33 ~ p.40
|
|
|
Antioxidative and Hepatocyte Protective Effects of Guava (Psidium guajava L.) Leaves Cultivated in Korea
|
|
Cheon Won-Young
Seo Dong-Yeon
Kim Young-Hwa
|
|
|
Abstract
|
|
|
|
The purpose of this study was to evaluate the antioxidant and hepatocyte protective effects of guava (Psidium guajava L.) leaves cultivated in Korea. The contents of the total polyphenol of the extract was 271.57 mg gallic acid equivalent (GAE)/ g residue. Antioxidant activities of leaf extract were evaluated by examining the free radical scavenging ability. 2,2'-azinobis-( 3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and α-α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging activities of the extract were 1133.23 mg trolox equivalent antioxidant capacity (TEAC)/g residue and 721.68 mg TEAC/g residue, respectively. The hepatocyte protective effect of guava leaf extract was examined in HepG2 cells. Against tert-butyl hydroperoxide (TBHP), the viability of HepG2 cells were increased by the treatment of leaf extract. In addition, guava leaf extract led to the inhibition of reactive oxygen species (ROS) generated in HepG2 cells. The leaf extract increased the activity of glutathione (GSH), glutathione reductase (GR), and glutathione peroxidase (GPx) against oxidative stress. These results suggested that guava leaves might be regarded as a potential source natural antioxidant and a hepatoprotective material.
|
|
|
KEYWORD
|
|
|
guava (Psidium guajava L.) leaf, antioxidative activity, hepatocyte protection, reactive oxygen species, antioxidant enzymes
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|